Coding

Part:BBa_K1964000

Designed by: Patrick Gerlinger   Group: iGEM16_Marburg   (2016-10-14)


[PanD] Aspartate 1-decarboxylase from C. glutamicum [Codon optimized]

Codon optimized version of the panD gene from C. glutamicum. Encodes for the aspatarte 1-decarboxylase that catalyzes the chemical reaction of L-aspartate to beta-alanine + CO2. The protein consists 136 amino acid residues with a deduced molecular mass of 14.1 kDa. It belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds.


panD was successfully integrated into the operon plasmid that was designed as part of our project. The integration was confirmed via sequencing.

The operon plasmid that was designed as part of our project to establish a dependency between an invading E. coli cell and its host S. cerevisisae

The operon plasmid leads to an significant incrase of malonate in E. coli Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None